✦ LIBER ✦

Regeneration of plants from callus tissue of the pasture legumeCentrosema brasilianum

✍ Scribed by P. N. Angeloni; H. Y. Rey; L. A. Mroginski

Publisher: Springer
Year: 1992
Tongue: English
Weight: 608 KB
Volume: 11
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00236268

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✦ Synopsis

Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and BAP, respectively. Shoots formed roots upon transfer to MS with 0.01 mg/l NAA. Plantlets were succesfully transferred to soil. Leaf-derived calli of Centrosema arenarium, C. macrocarpum, C. pascuorum, C. pubescens, and C. virginianum did not produce shoots when cultured in vitro.

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