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Plant regeneration from leaf-derived callus cultures of the tropical pasture legumeStylosanthes scabraVog.

โœ Scribed by Ian D. Godwin; Geoffrey H. Gordon; Donald F. Cameron

Publisher
Springer
Year
1987
Tongue
English
Weight
297 KB
Volume
9
Category
Article
ISSN
0167-6857

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โœฆ Synopsis

Callus cultures of 5 genotypes of S. scabra Vog. were optimally established from leaf tissue on Murashige and Skoog (MS) basal medium supplemented with 0.5-2.0mg 1-2, 4-Dichlorophenoxy acetic acid (2, 4-D) and 1.0-2.0 mg 1-1 6-benzylaminopurine (BAP). On media containing 2, 4-D only, calli were soft, and rhizogenesis occurred on calli of 4 genotypes. Calli formed on media containing BAP only, but not with kinetin only. In the presence of 2, 4-D, BAP inhibited rhizogenesis and promoted better callus growth than kinetin. High frequency shoot induction was achieved for 3 genotypes on MS + 2.0mgl -~ BAP. Roots formed on shoots when sub-cultured on half-strength MS without growth regulators. The form of cytokinin used in the callus induction media appeared to affect subsequent shoot organogenesis. Genotypic differences were observed for shoot organogenesis. There was some morphological variation evident among regenerants.

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