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REDUCTIVE ALKYLATION OF PROTEINS WITH AROMATIC ALDEHYDES AND SODIUM CYANOBOROHYDRIDE

โœ Scribed by Friedman, Mendel ;Williams, L. David ;Masri, M. Sid

Book ID
115097111
Publisher
Wiley (Blackwell Publishing)
Year
2009
Tongue
English
Weight
195 KB
Volume
6
Category
Article
ISSN
0367-8377

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Radiolabeling of proteins by reductive a
Radiolabeling of proteins by reductive alkylation with [14C]formaldehyde and sodium cyanoborohydride
โœ Diane Dottavio-Martin; Joanne M. Ravel ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 244 KB

A procedure is described for radiolabeling proteins in vitro by reductive alkylation. The proteins are treated with [W]formaldehyde in the presence of sodium cyanoborohydride, a reducing agent that is stable in aqueous solution at pH 7. The advantage of this procedure is that the reaction can be car