A procedure is described for radiolabeling proteins in vitro by reductive alkylation. The proteins are treated with [W]formaldehyde in the presence of sodium cyanoborohydride, a reducing agent that is stable in aqueous solution at pH 7. The advantage of this procedure is that the reaction can be car
β¦ LIBER β¦
Immobilization of proteins by reductive alkylation with hydrophobic aldehydes
β Scribed by Hua-Lin Wu; Gary E. Means
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 353 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Radiolabeling of proteins by reductive a
Radiolabeling of proteins by reductive alkylation with [14C]formaldehyde and sodium cyanoborohydride
β
Diane Dottavio-Martin; Joanne M. Ravel
π
Article
π
1978
π
Elsevier Science
π
English
β 244 KB
Immobilization of proteins on oxidized c
β
Nancy L. Stults; Lisa M. Asta; Y.C. Lee
π
Article
π
1989
π
Elsevier Science
π
English
β 674 KB
Mild periodate oxidation of certain commercially available crosslinked agarose beads (Sepharose CL-4B and CL-6B) results in the generation of aldehydo groups which were useful for immobilization of amino compounds by reductive amination using pyridine borane. Consumption of periodate ion and product
Reductive alkylation of aldehyde tosylhy
β
E. Vedejs; W.T. Stolle
π
Article
π
1977
π
Elsevier Science
π
French
β 194 KB
During unsuccessful attempts to generate dianions from aldehyde tosylhydrazones, we have
Catalytic Reductive Alkylation of Second
β
Tomoya Mizuta; Satoshi Sakaguchi; Yasutaka Ishii
π
Article
π
2005
π
John Wiley and Sons
β 21 KB
π 1 views
Protein stabilization via hydrophilizati
β
N. S. Melik-Nubarov; V. V. Mozhaev; S. Ε ikΕ‘nis; Karel Martinek
π
Article
π
1987
π
Springer Netherlands
π
English
β 285 KB
Sodium Borohydride as the Only Reagent f
β
Jason C. Dunham; Adam D. Richardson; Robert E. Sammelson
π
Article
π
2006
π
John Wiley and Sons
β 24 KB
π 2 views