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Radiolabeling of proteins by reductive alkylation with [14C]formaldehyde and sodium cyanoborohydride

✍ Scribed by Diane Dottavio-Martin; Joanne M. Ravel

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 244 KB
Volume: 87
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90706-6

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✦ Synopsis

A procedure is described for radiolabeling proteins in vitro by reductive alkylation. The proteins are treated with [W]formaldehyde in the presence of sodium cyanoborohydride, a reducing agent that is stable in aqueous solution at pH 7. The advantage of this procedure is that the reaction can be carried out at neutral pH for extended periods of time and over a wide range of temperatures (O-37°C). Moreover, owing to the flexibility in the reaction conditions afforded by the use of sodium cyanoborohydride, higher incorporation of radiolabel into protein can be attained.

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