✦ LIBER ✦

Recovery of protein by Coomassie Brilliant Blue precipitation prior to electrophoresis

✍ Scribed by Dr. Thomas Marshall; Katherine M. Williams

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 218 KB
Volume: 13
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.11501301195

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Protein concentration by precipitation w

Protein concentration by precipitation with pyrogallol red prior to electrophoresis

✍ Dr. Thomas Marshall; Nicola J. Abbott; Peter Fox; Katherine M. Williams 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 434 KB

Effect of Vanadate on Protein Determinat

Effect of Vanadate on Protein Determination by the Coomassie Brilliant Blue Microassay Procedure

✍ M. Matejovicova; K. Mubagwa; W. Flameng 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 79 KB

Electrophoresis in the presence of Cooma

Electrophoresis in the presence of Coomassie brilliant blue R-250 stains polyacrylamide gels during protein fractionation

✍ Julian Borejdo; Carroll Flynn 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 489 KB

A method of staining polyacrylamide gels in which the dye is electrophoresed together with the sample is proposed. The method cuts short and simplifies the conventional electrophoresis procedure by eliminating the separate poststaining step. In the gels run in the presence of sodium dodecyl sulfate,

Quantitation of proteins bound to polyvi

Quantitation of proteins bound to polyvinylidene difluoride membranes by elution of Coomassie brilliant blue R-250

✍ Steven R. Kain; Helen L. Henry 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 590 KB

A rapid and simple method for the quantitation of stained proteins bound to polyvinylidene difluoride (PVDF) membranes via the elution of Coomassie brilliant blue R-250 is described. A mixture of standard proteins was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrob

Simultaneous staining of proteins during

Simultaneous staining of proteins during polyacrylamide gel electrophoresis in acidic gels by countermigration of Coomassie brilliant blue R-250

✍ George Varghese; A.M. Diwan 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 739 KB

A method for the simultaneous staining of proteins during polyacrylamide gel electrophoresis with Coomassie brilliant blue R-250 at pH 2.5 is described. Calf thymus whole histone and cytochrome c were stained by this method and the results obtained were similar to that obtained by staining after ele

Quantitation of proteins by elution of C

Quantitation of proteins by elution of Coomassie brilliant blue R from stained bands after sodium dodecyl sulfate-polyacrylamide gel electrophoresis

✍ Eric H. Ball 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 424 KB

A simple method for the extraction of Coomassie brilliant blue R from stained protein bands excised from polyacrylamide gels is described. Spectrophotometric measurement of the eluted dye forms the basis of a sensitive assay to quantitate proteins in gels in the range 0.5-10 micrograms. The method r