Recovery of poly-3-hydroxybutyrate from recombinant Escherichia coli by homogenization and centrifugation
โ Scribed by Y. Ling; D.R.G. Williams; C.J. Thomas; A.P.J. Middelberg
- Book ID
- 110370112
- Publisher
- Springer-Verlag
- Year
- 1997
- Tongue
- English
- Weight
- 201 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0951-208X
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๐ SIMILAR VOLUMES
A simple method for the recovery of microbial poly(3-hydroxybutyrate) [P(3HB)] from recombinant Escherichia coli harboring the Ralstonia eutropha PHA biosynthesis genes was developed. Various acids (HCl, H 2 SO 4 ), alkalies (NaOH, KOH, and NH 4 OH), and surfactants (dioctylsulfosuccinate sodium sal
Several recombinant Escherichia coli strains, including XLI-Blue, JM109, HB101, and DH5a harboring a stable high-copynumber plasmid pSYL105 containing the Alcaligenes eutrophus polyhydroxyal ka noate (PHA) biosynt hesis genes were constructed. These recombinant strains were examined for their abilit