Recovery of agarose for electrophoresis of DNA fragments
✍ Scribed by Eduardo A. Ceccarelli; Néstor Carrillo
- Book ID
- 113289005
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 136 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0168-9525
No coin nor oath required. For personal study only.
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## Discontinuous buffer system for polyacrylamide and agarose gel electrophoresis of DNA fragments DNA fragments up to 9 kb in size were stacked and separated by polyacrylamide gel electrophoresis, and those up to 50 kb in size by agarose gel electrophoresis, using a discontinuous buffer system. P
A simple and reliable method for the recovery of specific fragments of DNA from agarose gels is presented. The electroelution of the DNA onto the NENSORB cartridge matrix with the subsequent elution of the bound DNA by a methanol (50% v/v) wash has been shown to result in the quantitative recovery o