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Agarose Gel Electrophoresis for the Separation of DNA Fragments

✍ Scribed by Lee, Pei Yun (author);Costumbrado, John (author);Hsu, Chih Yuan (author);Kim, Yong Hoon (author)


Book ID
115503991
Publisher
Journal of Visualized Experiments
Year
2012
Tongue
English
Weight
345 KB
Volume
62
Category
Article
ISSN
1940-087X

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## Discontinuous buffer system for polyacrylamide and agarose gel electrophoresis of DNA fragments DNA fragments up to 9 kb in size were stacked and separated by polyacrylamide gel electrophoresis, and those up to 50 kb in size by agarose gel electrophoresis, using a discontinuous buffer system. P