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Recombination deficient mutants of Streptococcus pyogenes K56

✍ Scribed by Dr. H. Malke

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
468 KB
Volume
15
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

Nitrosoguanidine mutagenesis of the Group A streptococcal strain K56 known as a good recipient in phage‐mediated transduction led to the isolation of two recombination deficient mutants showing transduction frequencies of chromosomal markers about 100‐fold lower than those of the parent strain. These mutants were sensitive to ultraviolet (UV) irradiation but had retained their ability to reactivate UV‐irradiated phage A25. They were cross‐sensitive to the killing action of nitrosoguanidine and ethyl methane sulfonate and showed severely inhibited DNA synthesis after UV radiation.

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