Using nitrosoguanidine (NTG) two nodulation defective (Nod-), auxotrophic mutants of the Rhizobium meliloti strain L5.30 have been isolated. In their Sym plasmid, pRmeLS.3Ob, a large deletion has been de- monstrated. Both mutants were adsorbed into root hairs of lucerne, but were unable to induce ro
Recombination deficient mutants of Rhizobium meliloti 41
✍ Scribed by Olasz, Ferenc ;Dorgai, László ;Páy, Anikó ;Orosz, László
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 344 KB
- Volume
- 191
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
Two mutants deficient in homologous genetic recombination have been isolated from Rhizobium meliloti 41 after Tn5 mutagenesis. Both mutants are defective in the induction of temperate phage 16-3 by UV-light, Mytomycin-C or Bleomycin, their UV sensitivity is more pronounced than that of the wild-type strain, and they lack the 'SOS activity' responsible for induced mutations.
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The inoculation of lucerne seedlings with mutants of Rhizohiuni rnelilofi (Arg-) requiring arginine resulted in the formation of ineffective nodules. In these nodules bacteria were not released from the infection threads into the nodule cytoplasm. When arginine was provided as a nitrogen source, the
## Abstract Nitrosoguanidine mutagenesis of the Group A streptococcal strain K56 known as a good recipient in phage‐mediated transduction led to the isolation of two recombination deficient mutants showing transduction frequencies of chromosomal markers about 100‐fold lower than those of the parent