𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Mutants of Rhizobium meliloti defective in nodulation

✍ Scribed by Dr. Wanda Małek

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
412 KB
Volume
28
Category
Article
ISSN
0233-111X

No coin nor oath required. For personal study only.

✦ Synopsis

Using nitrosoguanidine (NTG) two nodulation defective (Nod-), auxotrophic mutants of the Rhizobium meliloti strain L5.30 have been isolated. In their Sym plasmid, pRmeLS.3Ob, a large deletion has been de- monstrated. Both mutants were adsorbed into root hairs of lucerne, but were unable to induce root hair curlings. The nodulation of lucerne was restored to both hair-curling (Hac-) mutants by the introduction of the Sym plasmid pJB5JI of R. leguminosarum and by plasmid pEK25, carrying a 3.6 kb AvaI/EcoRI fragment of "common" nod genes of R. meliloti 41. This led to the conclusion that in these Nod-deletion mutants nod mutations were concerned with "common" nod genes.

📜 SIMILAR VOLUMES

Microscopic structure of ineffective alf
Microscopic structure of ineffective alfalfa nodules formed by auxotrophic mutants of Rhizobium meliloti
✍ Dr. Wanda Małek 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 599 KB

The inoculation of lucerne seedlings with mutants of Rhizohiuni rnelilofi (Arg-) requiring arginine resulted in the formation of ineffective nodules. In these nodules bacteria were not released from the infection threads into the nodule cytoplasm. When arginine was provided as a nitrogen source, the

Recombination deficient mutants of Rhizo
Recombination deficient mutants of Rhizobium meliloti 41
✍ Olasz, Ferenc ;Dorgai, László ;Páy, Anikó ;Orosz, László 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 344 KB

Two mutants deficient in homologous genetic recombination have been isolated from Rhizobium meliloti 41 after Tn5 mutagenesis. Both mutants are defective in the induction of temperate phage 16-3 by UV-light, Mytomycin-C or Bleomycin, their UV sensitivity is more pronounced than that of the wild-type