Characterization of a large plasmid of Rhizobium meliloti involved in enhancing nodulation
✍ Scribed by Toro, Nicolás ;Olivares, José
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 583 KB
- Volume
- 202
- Category
- Article
- ISSN
- 0026-8925
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RP4-prime plasmids containing chromosomal fragments of either Escherichia coli or Rhizobium meliloti were constructed in vitro. When introduced into E. coli or R. meliloti respectively, they promoted a polarized transfer of the chromosome as demonstrated either by the gradient of transfer of various
The Rhizobiwn legutninosariint bv. trrfolii Sym plasmid pRtr5a was transferred by conjugation to R . nieliloti strains deleted or mutated in the nod and.fir genes, and also to a plasmid-less strain of Agrobacterium runie/ucieris. The plasmid transfer led to the elimination of the resident cryptic pl