## Abstract Tobacco has proven to be a promising alternative for the production of recombinant therapeutic proteins and offers numerous advantages over other plants as a host system. However, the recovery and purification steps needed to obtain a protein at high recovery and purity have not been we
Recombinant protein purification from pea
โ Scribed by Todd J. Menkhaus; Cynthia Pate; Anthony Krech; Charles E. Glatz
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 276 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0006-3592
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Prokaryotic expression of polypeptides as fusion proteins with glutathione-S-transferase has recently been reported as a one-step means of purifying recombinant protein. The usefulness of the glutathione-S-transferase/glutathione- agarose system, however, is significantly limited by the frequent syn
The Saccharornyces cerevisiae strain Mc16/p520 has an unstable plasmid, p520; which directs production of a wheat a-amylase. The effects of immobilizing this microorganism on the plasmid stability and the specific productivity of the secreted a-amylase were investigated. Small gelatin beads were use