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Ligand Choice in Purifications of Recombinant Proteins by Expanded Bed Adsorption

✍ Scribed by Robert H. Clemmitt; Norhafizah Abdullah; Howard A. Chase

Publisher: John Wiley and Sons
Year: 2001
Tongue: German
Weight: 71 KB
Volume: 73
Category: Article
ISSN: 0009-286X
DOI: 10.1002/1522-2640(200106)73:6<721::aid-cite7212222>3.0.co;2-#

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## Abstract Expanded bed adsorption chromatography is used to capture products directly from unclarified feedstocks, thus combining solid–liquid separation, product concentration and preliminary purification into a single step. However, when non‐specific ion‐exchangers are used as the adsorbent in