✦ LIBER ✦

Increased protein productivity from immobilized recombinant yeast

✍ Scribed by Edward L. Walls; John L. Gainer

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 632 KB
Volume: 37
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260371107

No coin nor oath required. For personal study only.

✦ Synopsis

The Saccharornyces cerevisiae strain Mc16/p520 has an unstable plasmid, p520; which directs production of a wheat a-amylase. The effects of immobilizing this microorganism on the plasmid stability and the specific productivity of the secreted a-amylase were investigated. Small gelatin beads were used as the support in both fluidized and packed bed configurations, and the yeast cells were attached by covalent cross-linking with glutaraldehyde. These data were then compared to those for nonimmobilized, suspension cells.

Plasmid stability was increased for the immobilized cells during continuous culture at dilution rates both above and below washout. Continuous suspension cultures were not stable and rapidly lost the plasmid. Immobilization caused an increase in specific and volumetric productivity during continuous culture, with a packed bed design resulting in the highest specific productivity.

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