Studies on fermentation with recombinant yeast cells: plasmid stability and kinetics of biomass and protein production

✍ Scribed by Gülnur Birol; Zİlsen Önsan; Betül Kırdar

• Publisher: Wiley (John Wiley & Sons)
• Year: 2000
• Tongue: English
• Weight: 143 KB
• Volume: 75
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/1097-4660(200008)75:8<729::aid-jctb268>3.0.co;2-x

✦ Synopsis

Plasmid stability of the recombinant Saccharomyces cerevisiae YPB-G strain harbouring a YEp plasmid with a-amylase and glucoamylase genes as a fusion has been investigated in shake ¯asks and in a bioreactor using various compositions of media containing glucose or starch as the main carbon source. The medium composition affected both the growth characteristics and the stability of the plasmid. Superior plasmid stability was obtained in yeast minimal medium and in complex medium with glucose. Plasmid stability was substantially increased at high growth rates. Additional data were collected in the same system to investigate the kinetic characteristics of biomass and protein production, and unstructured kinetic models were used to interpret the results. At high initial glucose concentrations, where the biomass and protein production rates were similar, the kinetic models displayed good ®ts associated with high degrees of correlation.