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Real-time PCR-based determination of gene copy numbers in Pichia pastoris

✍ Scribed by Sandra Abad; Kerstin Kitz; Astrid Hörmann; Ulrike Schreiner; Franz S. Hartner; Anton Glieder

Book ID
111960581
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
203 KB
Volume
5
Category
Article
ISSN
1860-6768

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✍ Klaus Wilke; Bülent Duman; Jürgen Horst 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 261 KB

We report the development of a method for diagnosis of heterozygous deletions or duplications based on measurement of gene copy number. The method involves amplifications of a test locus with unknown copy number and a reference locus with known copy number using real-time PCR. Progress of the PCR re