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Improving Phytase Expression by Increasing the Gene Copy Number of appA-m in Pichia pastoris

โœ Scribed by LUO, H; HUANG, H; BAI, Y; WANG, Y; YANG, P; MENG, K; YUAN, T; YAO, B


Book ID
122798080
Publisher
Elsevier Science
Year
2006
Weight
426 KB
Volume
22
Category
Article
ISSN
1872-2075

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Seven transformants having varying numbers of non-homologously integrated copies of the isocitrate lyase gene, acu-7, were analysed for enzyme activity. Maximum levels of activity, 3.8 times that of the wild type, were observed in a transformant with only two gene copies whereas eight gene copies in