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Effect of gene copy number on production yield of recombinant ergopeptine hydrolase ErgA in Pichia pastoris

✍ Scribed by Panhölzl, Julia; Lindenberger, Julia; Menczik, Patricia; Aleschko, Markus; Hahn, Irene; Schwartz-Zimmermann, Heidi; Schatzmayr, Gerd; Thamhesl, Michaela; Moll, Wulf-Dieter


Book ID
125803998
Publisher
Elsevier
Year
2014
Tongue
English
Weight
60 KB
Volume
31
Category
Article
ISSN
1871-6784

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## Abstract __Pichia pastoris,__ a methylotrophic yeast, is an efficient producer of recombinant proteins in which the heterologous gene is under the control of the methanol‐induced __AOX1__ promoter. Hence, the accepted production procedure has two phases: In the first phase, the yeast utilizes gl