## Abstract Quantitative and sensitive imaging of chemiluminescence, bioluminescence and fluorescence emissions is emerging as an increasingly important technique for a range of biomedical applications (Hooper __et al__., 1990). A brief review of low‐light‐level imaging is presented, with particula
Real-time imaging of gene expression in single living cells
✍ Scribed by Guy A. Rutter; Helen J. Kennedy; Christopher D. Wood; Michael R.H. White; Jeremy M. Tavaré
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 718 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1074-5521
No coin nor oath required. For personal study only.
✦ Synopsis
Recent advances
in reporter gene technologies are now allowing us to image gene transcription at the single cell level, using either fluorescence or luminescence microscopy.
Here, the basis of these techniques is outlined and their advantages and disadvantages in various biological systems are discussed.
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Abbreviations ## FACS fluorescence-activated cell sorting GABA γ-aminobutyric acid LCM laser capture microdissection PCR polymerase chain reaction RT reverse-transcriptase
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