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Single-Molecule Imaging of Signaling Molecules in Living Cells

✍ Scribed by Yasushi Sako; Kayo Hibino; Takayuki Miyauchi; Yoshikazu Miyamoto; Masahiro Ueda; Toshio Yanagida

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
599 KB
Volume
1
Category
Article
ISSN
1438-5163

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Using a setup based on fluorescence microscopy we study fluorescence spectra of single chromophores. Being point like emission sources single molecules can be focused to the entrance slit of a spectrometer. Even with narrow slit width the light passes the system with almost no loss. We show that, ma