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Long-term, noninvasive imaging of regulated gene expression in living mice

✍ Scribed by Mazahir T. Hasan; Kai Schönig; Stefan Berger; Walter Graewe; Hermann Bujard

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
570 KB
Volume
29
Category
Article
ISSN
1526-954X

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✦ Synopsis

Abstract

Summary: We describe here an approach for monitoring regulated gene expression by noninvasive imaging in living mice. We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, respectively. Results from our model system demonstrate that luciferase can be used as a noninvasive imaging marker for the regulated expression of a second gene in living mice. The integration of noninvasive imaging and inducible gene expression into current approaches of functional genomics should greatly advance our capabilities of carrying out highly controlled long‐term studies of gene function in individual mice. genesis 29:116–122, 2001. © 2001 Wiley‐Liss, Inc.

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