𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Long-term tracing of adenoviral expression in rat and rabbit using luciferase imaging

✍ Scribed by Jin Zhong Li; David Holman; Hongwei Li; An-Hsiang Liu; Brian Beres; Gerald R Hankins; Gregory A Helm

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
386 KB
Volume
7
Category
Article
ISSN
1099-498X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background

Luciferase optical imaging provides a novel method to monitor transgene expression in small living animals. As the genetic and immunological heritages of particular animals significantly affect the expression of adenovirus‐delivered transgenes, it is essential to know the expression patterns specific to athymic nude and Sprague‐Dawley rats, two strains commonly used in rodent models. In this study we set out to determine these patterns. At the same time, we tested luciferase optical imaging in a larger animal, the rabbit.

Methods

A recombinant luciferase adenoviral vector was injected subcutaneously or intramuscularly into athymic nude rats, Sprague‐Dawley rats, and Dutch Belted rabbits. The luciferase expression was assessed using a cooled charge‐coupled device.

Results

The luminescent signal was capable of passing through at least 1.3 cm of muscle tissue and proved to be much stronger when luciferin was delivered via a local injection than by an intraperitoneal injection. Although the types of immune cells differed between immunodeficient and immunocompetent rats, similar amounts and patterns of luciferase expression were observed in the musculature in two rat strains during the 1st month after a viral intramuscular injection. The duration of luciferase expression was longer than 15 months in athymic nude rats, 9 months in Sprague‐Dawley rats, and 6 months in rabbits following a direct viral injection.

Conclusions

Luciferase expression after adenoviral gene delivery can persist for longer than 6 months, even in immunocompetent animals. Live imaging of luciferase expression can be performed not only in small animals, but also in larger animals such as rabbits. Copyright © 2005 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Extended-release PEG–luciferin allows fo
Extended-release PEG–luciferin allows for long-term imaging of firefly luciferase activity in vivo
✍ Sachin S. Chandran; Simon A. Williams; Samuel R. Denmeade 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 258 KB

## Abstract Bioluminescence has gained favour in the last decade as an approach for observing tumours __in vivo__ in a non‐destructive manner. This very sensitive technique is based on light emission by the reaction of luciferin with the enzyme luciferase, as measured by a photodetector. Ever since

Long-term, noninvasive imaging of regula
Long-term, noninvasive imaging of regulated gene expression in living mice
✍ Mazahir T. Hasan; Kai Schönig; Stefan Berger; Walter Graewe; Hermann Bujard 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 570 KB

## Abstract Summary: We describe here an approach for monitoring regulated gene expression by noninvasive imaging in living mice. We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, resp

Long-term transgene expression in cardia
Long-term transgene expression in cardiac and skeletal muscle following fetal administration of adenoviral or adeno-associated viral vectors in mice
✍ Sarah Bouchard; Tippi C. MacKenzie; Antoneta P. Radu; Satoshi Hayashi; William H 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 358 KB

## Abstract ## Background __In utero__ gene transfer may provide advantages for the correction of congenital genetic disorders. In the present study we compare the ability of adenovirus (AdCMVLacZ), and two serotypes of adeno‐associated virus (AAVCMVLacZ serotypes 2 and 2/5), to target cardiac and

Long-term evaluation of bone-titanium in
Long-term evaluation of bone-titanium interface in rat tibiae using light microscopy, transmission electron microscopy, and image processing
✍ Takeshita, Fumitaka ;Ayukawa, Yasunori ;Iyama, Shinji ;Murai, Kenji ;Suetsugu, T 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 378 KB 👁 2 views

We conducted a 2-year histologic and histometric a poorly mineralized layer remained in some areas between evaluation of the tibial bone-titanium (Ti) implant interface the mature bone and the titanium, and the interposed amorin male rats. Thirty male 6-week-old rats were used in this phous zone was