Reagents suitable for the crosslinking of nucleic acids to proteins

✍ Scribed by G. Fink; H. Fasold; W. Rommel; R. Brimacombe

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 692 KB
• Volume: 108
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90604-1

✦ Synopsis

The synthesis of 3-(4-bromo-3-oxobutane-I-sulphonyl)propionic acid p-nitrophenyl ester, of the corresponding N-hydroxysuccinimide ester, and of p-azidophenylacetic imidoester is described. The first two compounds may be attached to adenine and cytosine in nucleic acid-protein mixtures at pH 6.0 preferentially, although in competition with side reactions to amino acid side chains, and then to protein in a second step at pH 8. The third compound rapidly reacts with protein lysine amino groups at pH 7.8, and, after photoactivation of the azido group, shows a preference in the second step reaction for the aromatic bases of the nucleic acid in the mixture.