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Ethidium dimer: A new reagent for the fluorimetric determination of nucleic acids

โœ Scribed by Judith Markovits; Bernard P. Roques; Jean-Bernard Le Pecq

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
417 KB
Volume
94
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A dimeric derivative of ethidium is used for fluorimetric assay of nucleic acids. Due to the very high binding affinity of this derivative for DNA and RNA, a significant increase of sensitivity of the ethidium fluorimetric technique for nucleic acids determination is obtained. Using a commercially available instrument, DNA concentrations in the nanogram per milliliter range are determined. In addition, we have found that an acridine ethidium dimer can also be used for a sensitive fluorimetric assay of DNA concentration, while simultaneously providing a determination of the DNA base composition.

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