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Ready identification of disulfide-bonded polypeptides by one-dimensional sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis

✍ Scribed by Asitha Gurusinghe; Patricia A. Ryan; Dr. Paul F. Davis

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 359 KB
Volume: 7
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.1150070208

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✦ Synopsis

Ready identification of disulfide-bonded polypeptides by one-dimensional sodium dodec yl sulfate-poly acr ylamide slab gel electrophoresis

A method for the detection of disulfide bonded polypeptide components in protein mixtures is described. During the electrophoresis of proteins in sodium dodecyl sulfate-polyacrylamide slab gels, the migration is interrupted and advantage is taken of the rapid diffusion of added 2-mercaptoethanol into lanes without reductant. In this paper, the results of applying this procedure to proteins known to contain only inter-chain (a,-macroglobulin, fibrinogen, type I11 collagen) and intrachain disulfides (bovine serum albumin, alkaline phosphatase) as well as to crude extracts are reported.

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