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Reactor type sensor for cyanide using an immobilized microorganism

✍ Scribed by Jeong Im Lee; Isao Karube

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
375 KB
Volume
8
Category
Article
ISSN
1040-0397

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✦ Synopsis

Abstract

A reactor type biosensor using an immobilized bacteria (Pseudomonas fluorescens NCIMB 11764) columm and an oxygen electrode was developed for cyanide. The optimum conditions for the sensor in a flow system (pH 9, 25Β°C, flow rate 2.5 mg/min) resulted in a response time of less than 5min. A linear relationship was observed between the current decrease and the cyanide concentration over the range of 0.05βˆ’1 mg/L. Operating at 25 Β°C, the sensor was stable up to 30 days. Alginate gel as the immobilized matrix was more suitable than other matrices (agars, polyurethane, porous glass beads, zeolite, chitopearl). Satisfactory results were obtained when the sensor was applied to determine the concentration of cyanide in the river water.

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