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Flow-type cyanide sensor using an immobilized microorganism

✍ Scribed by Kazunori Ikebukuro; Masahiro Honda; Keijiro Nakanishi; Yoko Nomura; Yuzo Masuda; Kenji Yokoyama; Yoshihiro Yamauchi; Isao Karube

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
419 KB
Volume
8
Category
Article
ISSN
1040-0397

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✦ Synopsis

A flow system for cyanide detection using Succhuromyces cerevisiue is described. This sensor system consists of two oxygen electrodes and immobilized S. cerevisiue whose respiration activity is measured by the oxygen electrodes. Since cyanide inhibits S. cerrvisiue's respiration, it can be detected by measuring this inhibition. Two types of systems were constructed, characterized and compared. One is a membrane-type flow system in which S. cerevisiue was immobilized onto a cellulose membrane. The other is a reactor-type flow system in which S. cerevisiue was immobilized onto the glass beads. The sensor response was examined in the range from 0 to 15pM. The reactor-type sensor system displayed lower detection limit (0.15 pM) and better stability (I6 days).

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