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Reactivity of Limulus amoebocyte lysate towards (1 → 3)-β-d-glucans

✍ Scribed by Naohito Ohno; Yutaka Emori; Toshiro Yadomae; Kazuo Saito; Akiyoshi Masuda; Shozo Oikawa

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
520 KB
Volume
207
Category
Article
ISSN
0008-6215

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✦ Synopsis

The structure activity relationship for beta-D-glucans for the gelation of the amoebocyte lysates of the horseshoe crab (Limulus) has been investigated. beta-D-Glucans that had no (1----3) linkages induced little or no gelation. The (1----3)-beta-D-glucans curdlan (unbranched), grifolan (approximately 33% branched), schizophyllan (approximately 33% branched), lentinan (approximately 40% branched). SSG (approximately 50% branched), and OL-2 (approximately 66% branched) induced significant gelation. The optimum concentration for gelation was correlated with the content of branching. Single chain (rather than a triple helix) conformation and higher molecular weight were associated with higher reactivity.

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