(1↠3)-β-D-Glucan in culture fluid of fungi activates factor g, a limulus coagulation factor

✍ Scribed by Dr. Takashige Miyazaki; Shigeru Kohno; Kotaro Mitsutake; Shigefumi Maesaki; Ken-Ichi Tanaka; Kohei Hara

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 521 KB
• Volume: 9
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860090509

✦ Synopsis

Two well-known polysaccharides, (133)-activated the limulus factor G! and that not B-D-glucan and mannan, are major Structural only the conidia form but also the filamencomponents of the fungus cell wall. The G tous form of Aspergillus fumigatus reacted test is a direct method to measure (1+3)$-with factor G. Various Candida spp. (C. D-glucan using a (1 +3)-p-D-glucan-sensitive paraspilosis, C. glabrata, C. tropicalis, C. component, factor G, fractionated from the krusei) Saccharomyces cerevisiae, limulus lysate. The concentration of (1 -3)-

Rhodotorula rubra, Trichosporon beigelii, P-D-glucan in culture Supernatants of Canand A. fumigafus released soluble (1 +3)-pdida albicans increased to 1,390.0 p g h l at 0-glucan into their culture supernatants, but 24 hours. The concentration of mannan also C. neoformans and Cunninghamella increased parallel with fungal growth. How-bertholletiae showed only a small reaction ever, after digestion of Supernatants with to the G test during their culture. Our reendo-(1 -+3)-p-D-glucanase, the reactivity to sults indicate that the G test is a good factor G disappeared, although titers of anti-method for serodiagnosis of deep mycosis mannan monoclonal antibody-based latex and also as a screening tool for contamiagglutination were unchanged. Our study nation of medical devices, drugs, and exdemonstrated that cell suspensions of both perimental materials with (1 3 3 ) -