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Degradation of a glucan containing β-(1→3) and β-(1→6) linkages by exo-(1→3)-β-d-glucanase

✍ Scribed by Ryoichi Yamamoto; Donald J. Nevins

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
629 KB
Volume
122
Category
Article
ISSN
0008-6215

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✦ Synopsis

A P-D-glucan isolated from a Japanese marine alga known as "Arame" (Eisenia bicyclis) was shown by methylation analysis to be composed of (l-3) and (l-+6) linkages and ( 1+3),(1+6) branch points. The 13C-n.m.r. spectrum confirmed these linkages and also verified the absence of /3-( l-+4) linkages. The endo-(1-3)~P-D-glucanase of Rhizopus liberates from the native glucan oligosaccharides having an odd number of glucosyl residues, such as 3, 5,7, and 9. The exo-(l-3)-/?-D-glucanase of Basidiomyete QM 806 produces P-glucose, gentiobiose, and 6-0-&laminarabiosyl-D-glucose, and a tetrasaccharide, /3-D-Glc-(l-+6)-P-D-Glc-(1+3)-P-D-Glc-(l-+6)-D-Glc.

Thus, the exo-enzyme appears to bypass glucosyl substituents at O-6 of 3-O-linked glucose in this polymer and, because it liberates oligosaccharides, it behaves as if it mediates an endo-type hydrolysis of the glucan.

D-glucosyl groups

at O-6 of every third glucose residue in the main chain4,5. This degradation pattern suggests that the enzyme circumvents the p-(1-+6) linkages, as they appear at the terminal end, and at-

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