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Reaction Mechanism for the Diastereocontrolled Synthesis of Phosphorothioate DNA by the Oxazaphospholidine Approach

✍ Scribed by Takeshi Wada; Natsuhisa Oka; Kazuhiko Saigo


Publisher
John Wiley and Sons
Year
2004
Weight
61 KB
Volume
35
Category
Article
ISSN
0931-7597

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Easily accessible phenacetyl or benzoyl disulfide proved to be very convenient reagents for a rapid Psulfurttation of phosphfte-triesters and H-phosphonate diesters, respectively. Phosphorothioate (PS) analogues of nucleotides are useful probes to study phosphoryl and nucleotidyl transferring enzyme