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ras Gene mutations and hpv infection are common in human laryngeal carcinoma

✍ Scribed by Khurshid Anwar; Kazuya Nakakuki; Hironobu Naiki; Manabu Inuzuka

Publisher: John Wiley and Sons
Year: 1993
Tongue: French
Weight: 1011 KB
Volume: 53
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910530106

No coin nor oath required. For personal study only.

✦ Synopsis

Kazuya NAKAKUKI', Hironobu NAIKI' and Manabu INUZUKA~ To evaluate the role of ras activation and human papillomavirus (HPV) infection in laryngeal carcinoma, we analyzed tumor DNA from 43 cases, including 25 primary laryngeal tumors, I 2 lymph-node and one skin metastases, and 5 recurrent laryngeal carcinomas. Thirteen normal laryngeal tissues and 7 benign laryngeal nodule biopsy specimens along with normal tissue surrounding laryngeal carcinoma in 2 cases were also included. The polymerase-chain-reaction technique was used to amplify DNA fragments containing codon I 2 and 6 I of H-, Kand N-ras, also HPV 16, 18 and 33 DNA, subsequently hybridized with sequence-specific oligonucleotides. DNA samples from 22 patients with laryngeal carcinoma revealed ras mutations (18 in N-ras codon 12, 6 in H-ras codon 6 I, and 3 in K-ras codon 6 I). Likewise, HPV DNA was found in I6 cases (HPV 16,18 and 33 in 3 cases, 14 cases and I case respectively). ras mutations were significantly higher in metastatic tumors (I 0 of I 3 cases) than in primary (I I of 25 cases) and recurrent laryngeal carcinomas (I of 5 cases). HPV DNA was detected in 60% of recurrent, 44% of primary and 15% of metastatic tumors. Only 2 of the I 3 normal laryngeal tissues and I out of 7 laryngeal nodule specimens were found to contain HPV DNA. These results suggest that ras activation, especially in N-ras codon 12. I (GGT + AGT) and HPV infection are 2 important factors in (multistage) laryngeal carcinogenesis. The ras mutation may be associated with metastatic ability of the tumor.

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