The technique of isoelectric focusing on methylated 'cellogel' strips (CAGIF) was used to confirm the presence of four alleles of PGM1 in human red cell lysates. The subtypes of PGM1 were determined in two Polish population samples, from Southwestern Poland (WrocΕaw region, n=321) and Southeastern P
Rare phenotypes of the phosphoglucomutase locus 1 detectable by isoelectric focusing on Cellogel
β Scribed by T. Dobsz; P. Koziol
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 578 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0340-6717
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β¦ Synopsis
The rare phenotypes PGM1, determined by alleles PGM1(3), PGM1(4), PGM1(6), and PGM1(7) were examined by starch gel electrophoresis and cellulose acetate gel isoelectric focusing and were compared with the commonest phenotypes of PGM1. The frequencies of the rare genes found in the Polish populations were as follows: in Lublin, PGM1(3) = 0.0002, PGM1(4) = 0.0005, PGM1(6) = 0.0010, and PGM1(7) = 0.0005; in WrocΕaw, PGM1(3) = 0.0000, PGM1(4) = 0.0005, PGM1(6) = 0.0007, and PGM1(7) = 0.0002. The results suggest that the F and S type variants of the genes PGM1(4) and PGM1(7) probably do not occur. It is still possible that F and S variants exist for the genes PGM1(3) and PGM1(6).
π SIMILAR VOLUMES
The existence of four alleles of phosphoglucomutase (PGM1) in human red cell lysates has previously been demonstrated by isoelectric focusing (Bark et al., 1976; KΓΌhnl et al., 1977; Sutton and Burgess, 1978). Experiments are now described in which the position of each of the first-locus (PGM1) and s
The phenotype distribution and gene frequencies of serum a~antitrypsin in 856 healthy blood donors in Tokyo were examined by gel slab isoelectric focusing (pH 4--6). The allele of the common subtype variant Pi M2 was present with a frequency of 0.1099 in Japanese. A study of 23 twin pairs and their
Phosphoglucomutase (PGM1) phenotypes were determined in a population sample of Tuscany, Italy, by isoelectric focusing. The frequencies observed for the four alleles are: PGM1+1 = 0.6012, PGM1-1 = 0.1059, PGM2+1 = 0.2495, PGM2-1 = 0.0434. Two variants were detected and it was possible to study the p