✦ LIBER ✦

Rapid yeast DNA staining method for flow cytometry

✍ Scribed by Dr. Doris Kuchenbecker; G. Braun

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 262 KB
Volume: 25
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620250812

No coin nor oath required. For personal study only.

✦ Synopsis

The variation of the fluorescence intcnsity of olivomycin-stained yeast cells as a function of the concentrdtion of olivomycin, NaCI, and MgCI, in the staining solution was studied.

The best results were obtained when the staining solution contained 100 pg olivomycin/ml, 40 mM MgC12, and 1 M NaCI. A staining time of 12 min was sufficient for proper staining.

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