Rapid determination of the purity of yeast cultures by flow cytometry
โ Scribed by K.-J. Hutter; H. E. Eipel; H. Hettwer
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 354 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Some features of a synchronized yeast culture detected by flow-cytometry
## Abstract __Legionella pneumophila__ labeled with specific fluorescent antibody was detected in a suspension of organisms by means of flow cytometry. The presence of other microorganisms in the suspension did not interfere with the recognition of Legionella when present at concentrations of 10^4^
The ability of a flow cytometer to rapidly assess microbial viability was investigated using three vital stains: rhodamine 123 (Rh123); 3,3'-dihexyloxacarbocyanine iodide [DiOC6(3)] and fluorescein diacetate (FDA). Rh123 was found to clearly differentiate viable from non-viable bacteria. The methodo