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Rapid, one step staining procedures for analysis of cellular DNA and protein by single and dual laser flow cytometry

โœ Scribed by H. A. Crissman; J. A. Steinkamp


Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
743 KB
Volume
3
Category
Article
ISSN
0196-4763

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We have developed a "one-tube" triple staining procedure that allows the identification of intratumor phenotypic subpopulations by FCM. Solid tumors were dissociated by a combined mechanid enzymatic method. Ovarian ascites tumor cell aggregates were enzymatically dissociated using trypsin. A n antik