The developed method of ion-pair reverse-phase high-pressure liquid chromatography (hplc) enables one to separate adenine nucleotides in cardiac tissue extract within 10 min with recovery of about 98% and lowest detection limit of 10m6 M. Separation of creatine and phosphocreatine is performed by io
Rapid separation of creatine, phosphocreatine and adenosine metabolites by ion-pair reversed-phase high-performance liquid chromatography in plasma and cardiac tissue
โ Scribed by Michael D. Scott; Lawrence J. Baudendistel; Thomas E. Dahms
- Publisher
- Elsevier Science
- Year
- 1992
- Weight
- 536 KB
- Volume
- 576
- Category
- Article
- ISSN
- 0378-4347
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The utilization of isocratic, reverse-phase, ion-paired high-performance liquid chromatography for analysis of creatine phosphate allows for rapid quantification of multiple samples. Cryogenic sample handling and the addition of ethylene glycol bis(j3-aminoethyl ether) NJ'-tetraacetic acid as a Ca2+
A sensitive and specific assay for measurement of adenine nucleotides and adenosine by paired-ion high-performance liquid chromatography is described. The I,N6-ethenoderivatives of ATP (c-ATP), ADP (+ADP), AMP (c-AMP). and adenosine (c-Ado), formed by reaction with chloroacetaldehyde at 37ยฐC. were s