Determiantion of creatine phosphate levels in brain tissue by isocratic reverse-phase, ion-paired high-performance liquid chromatography

✍ Scribed by P.M. McMahon; P.L. Lutz

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 240 KB
• Volume: 138
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90798-x

✦ Synopsis

The utilization of isocratic, reverse-phase, ion-paired high-performance liquid chromatography for analysis of creatine phosphate allows for rapid quantification of multiple samples. Cryogenic sample handling and the addition of ethylene glycol bis(j3-aminoethyl ether) NJ'-tetraacetic acid as a Ca2+ sequestering agent during perchloric acid extraction enhance maximal recovery of creatine phosphate from brain samples. Peak identification is supported by a complete enzymatic shift with a phosphocreatine kinase, hexokinase, and glucose-6-phosphate dehydrogenase system.