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Rapid assay of adenine nucleotides or creatine compounds in extracts of cardiac tissue by paired-ion reverse-phase high-performance liquid chromatography

✍ Scribed by E. Juengling; H. Kammermeier

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 271 KB
Volume: 102
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90167-0

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✦ Synopsis

The developed method of ion-pair reverse-phase high-pressure liquid chromatography (hplc) enables one to separate adenine nucleotides in cardiac tissue extract within 10 min with recovery of about 98% and lowest detection limit of 10m6 M. Separation of creatine and phosphocreatine is performed by ion-pair reverse-phase hplc (recovery about 98%) using detector wavelengths of 210nm; the lowest detection limit is in the range of 3 x 10e6 M.

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