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Rapid separation of acetylated oligosaccharides by reverse-phase high-pressure liquid chromatography

โœ Scribed by Gerald B. Wells; Robert L. Lester

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
547 KB
Volume
97
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Peracetylated saccharides were separated by chromatography on a reverse-phase support, eluting with mixtures of acetonitrile-water.

Gradient elution for 2.5 h gave significant separations of all linear glucose oligomers containing up to 35 sugar residues. With isocratic elution retention was exponentially related to molecular mass and only slightly affected by linkage or anomeric configuration. The presence of glucosamine in various saccharides markedly reduced their retention.

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