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Rapid screening and identification of cytochalasins by electrospray tandem mass spectrometry

✍ Scribed by Jeevan Kumar Prasain; Masashi Ueki; Piotr Stefanowicz; Hiroyuki Osada

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
219 KB
Volume
37
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

The cytochalasin class of fungal metabolites was analyzed by electrospray ionization tandem mass spectrometry (ESI‐MS/MS) with the aim of developing a methodology for their rapid identification in microbial extracts. ESI‐MS analyses of reference cytochalasins were performed and several product ions were produced in MS/MS experiments on parent ions that are structurally characteristic. A precursor ion search was performed to detect cytochalasins in an ethyl acetate extract of fungal strain RK97‐F21. Three cytochalasins were detected and one of the components was identified as epoxycytochalasin H by comparing the tandem mass spectra of the product ions with those of reference compounds. This finding was further validated by LC/MS and LC/MS/MS experiments. Copyright Β© 2002 John Wiley & Sons, Ltd.

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