𝔖 Bobbio Scriptorium
✦   LIBER   ✦

RAPID RELEASE OF PLASMIDS FROM LIVE OR DEAD ESCHERICHIA COLI CELLS

✍ Scribed by FAN FENG; SHULI WANG; ZHENLANG CHEN; QIUYUN LIU

Book ID
111344501
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
68 KB
Volume
14
Category
Article
ISSN
1060-3999

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A simplified procedure for the rapid ide
A simplified procedure for the rapid identification of recombinant pAT153 plasmids in Escherichia coli HB101 cells
✍ Michel Dion; Jocelyn Yelle; Claude Hamelin πŸ“‚ Article πŸ“… 1986 πŸ› Elsevier Science 🌐 English βš– 778 KB

Insertion of foreign DNA into the unique HindIII site of the high copy number plasmid pAT153 reduces but does not completely abolish the resistance of Escherichia coli HB101 cells to tetracycline. Recombinant DNA-containing colonies could then be phenotypically differentiated from non-recombinant on