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A RAPID PLASMID PREPARATION METHOD BY THE DIRECT BOILING OF ESCHERICHIA COLI CELLS

โœ Scribed by LAN OUYANG; LIANG QIN; ZHIWU XU; JIANGUO HE; QIUYUN LIU

Book ID
111344570
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
152 KB
Volume
16
Category
Article
ISSN
1060-3999

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A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15โ€“40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and ca

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A modification of the rapid boiling method for the preparation of bacterial plasmids allows it to be readily used in conjunction with standard CsCI-ethidium bromide density centrifugation for the-isolation of purified plasmids.