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Release of transforming plasmid DNA from actively growing genetically engineered Escherichia coli

✍ Scribed by Nobuyoshi Ishii; Kazuaki Matsui; Shoichi Fuma; Hiroshi Takeda; Zen’ichiro Kawabata


Book ID
108131138
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
246 KB
Volume
240
Category
Article
ISSN
0378-1097

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Sheared DNA from RPI, and R plasmid from a strain of Pseudomonas aeruginosa, was used to transform other strains of P. aeruginosa and Escherichia coli. From transformed cells other plasmids like RPI were isolated. These deletion plasmids were conjugally transferrable and confer resistance mainly aga