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Transformation of Pseudomonas aeruginosa and Escherichia coli with resistance plasmid DNA: Formation of smaller conjugative plasmids from RPI

✍ Scribed by Haque, Habibul

Publisher: Springer
Year: 1979
Tongue: English
Weight: 259 KB
Volume: 171
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00274021

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✦ Synopsis

Sheared DNA from RPI, and R plasmid from a strain of Pseudomonas aeruginosa, was used to transform other strains of P. aeruginosa and Escherichia coli. From transformed cells other plasmids like RPI were isolated. These deletion plasmids were conjugally transferrable and confer resistance mainly against carbenicillin and tetracycline.

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RSU2, a plasmid transmissible between strains of P. aeruginosa but not to Escherichia coli can be mobilized by R751. Conjugatants receive a single plasmid composed of DNA from both R751 and RSU2 which has the compatibility properties of a member of group P (like R751). Study of this fusion plasmid s