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Rapid One-Step Isolation of Mouse Liver Catalase by Immobilized Metal Ion Affinity Chromatography

โœ Scribed by Qian Yang; Joseph W. DePierre


Book ID
115645258
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
122 KB
Volume
12
Category
Article
ISSN
1046-5928

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Electrospray ionization mass spectrometry (ESI/MS) affords a rapid and sensitive technique for determining peptides produced by the enzymatic digestion of phosphoroteins. When coupled with on-line immobilized metal-ion affinity chromatography (IMAC), the combmation allows separation and mass spectro