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Current Protocols in Molecular Biology || Isolation of Phosphopeptides by Immobilized Metal Ion Affinity Chromatography

✍ Scribed by Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin


Book ID
120414539
Publisher
Wiley (John Wiley & Sons)
Year
2007
Tongue
English
Weight
316 KB
Volume
77
Category
Article
ISBN-13
9780471142720
ISSN
1934-3639

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Electrospray ionization mass spectrometr
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Electrospray ionization mass spectrometry (ESI/MS) affords a rapid and sensitive technique for determining peptides produced by the enzymatic digestion of phosphoroteins. When coupled with on-line immobilized metal-ion affinity chromatography (IMAC), the combmation allows separation and mass spectro