Rapid method for the determination of glutathione transferase isoenzymes in crude extracts
✍ Scribed by E. Marti´nez-Lara; P. Pascual; F. Toribio; J. Lo´pez-Barea; J.A. Ba´rcena
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 543 KB
- Volume
- 609
- Category
- Article
- ISSN
- 1873-3778
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ATP-sulfurylase (EC 2.7.7.4) catalyzes the first step in assimilatory sulfate reduction, forming adenosine 5'-phosphosulfate (APS) and pyrophosphate from ATP and SO:-. The extractable activity of ATP-sulfurylase was determined in crude extracts from Phaseolus vulgaris by measuring the formation of A
## Methods Reagents. Bz-Arg-NPhNO, was obtained from Seikagaku Kogyo Co., Ltd. Phenylmethylsulfonyl fluoride was purchased from Sigma. All other chemicals were reagent grade and commercially available. p-Dimethylaminobenzaldehyde reagent was prepared as follows: Concentrated sulfuric acid, 2.74 ml